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Construction of a Functional S-Layer Fusion Protein Comprising an Immunoglobulin G-Binding Domain for Development of Specific Adsorbents for Extracorporeal Blood Purification
The chimeric gene encoding a C-terminally-truncated form of the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and two copies of the Fc-binding Z-domain was constructed, cloned, and heterologously expressed in Escherichia coli HMS174(DE3). The Z-domain is a synthetic analogue of the B-domain...
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Main Authors: | , , , , , , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
American Society for Microbiology
2004
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC368406/ https://ncbi.nlm.nih.gov/pubmed/15006773 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.3.1514-1521.2004 |
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