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Molecular Characterization of the S-Layer Gene, sbpA, of Bacillus sphaericus CCM 2177 and Production of a Functional S-Layer Fusion Protein with the Ability To Recrystallize in a Defined Orientation while Presenting the Fused Allergen

The nucleotide sequence encoding the crystalline bacterial cell surface (S-layer) protein SbpA of Bacillus sphaericus CCM 2177 was determined by a PCR-based technique using four overlapping fragments. The entire sbpA sequence indicated one open reading frame of 3,804 bp encoding a protein of 1,268 a...

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Detalhes bibliográficos
Main Authors: Ilk, Nicola, Völlenkle, Christine, Egelseer, Eva M., Breitwieser, Andreas, Sleytr, Uwe B., Sára, Margit
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2002
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC126809/
https://ncbi.nlm.nih.gov/pubmed/12089001
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.68.7.3251-3260.2002
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