Construction of a Functional S-Layer Fusion Protein Comprising an Immunoglobulin G-Binding Domain for Development of Specific Adsorbents for Extracorporeal Blood Purification

The chimeric gene encoding a C-terminally-truncated form of the S-layer protein SbpA from Bacillus sphaericus CCM 2177 and two copies of the Fc-binding Z-domain was constructed, cloned, and heterologously expressed in Escherichia coli HMS174(DE3). The Z-domain is a synthetic analogue of the B-domain...

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Detalhes bibliográficos
Main Authors: Völlenkle, Christine, Weigert, Stefan, Ilk, Nicola, Egelseer, Eva, Weber, Viktoria, Loth, Fritz, Falkenhagen, Dieter, Sleytr, Uwe B., Sára, Margit
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Physiology and Biotechnology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC368406/
https://ncbi.nlm.nih.gov/pubmed/15006773
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.3.1514-1521.2004
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