Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family

We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had K(m) values for benzoate...

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Detalhes bibliográficos
Main Authors: He, Aimin, Li, Tao, Daniels, Lacy, Fotheringham, Ian, Rosazza, John P. N.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC368342/
https://ncbi.nlm.nih.gov/pubmed/15006821
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.3.1874-1881.2004
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