Nocardia sp. Carboxylic Acid Reductase: Cloning, Expression, and Characterization of a New Aldehyde Oxidoreductase Family

We have cloned, sequenced, and expressed the gene for a unique ATP- and NADPH-dependent carboxylic acid reductase (CAR) from a Nocardia species that reduces carboxylic acids to their corresponding aldehydes. Recombinant CAR containing an N-terminal histidine affinity tag had K(m) values for benzoate...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	He, Aimin, Li, Tao, Daniels, Lacy, Fotheringham, Ian, Rosazza, John P. N.
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2004
Fag:	Enzymology and Protein Engineering
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC368342/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/15006821/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.70.3.1874-1881.2004
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