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Construction of Chimeric Catechol 2,3-Dioxygenase Exhibiting Improved Activity against the Suicide Inhibitor 4-Methylcatechol

Catechol 2,3-dioxygenase (C23O; EC 1.3.11.2), exemplified by XylE and NahH, catalyzes the ring cleavage of catechol and some substituted catechols. C23O is inactivated at an appreciable rate during the ring cleavage of 4-methylcatechol due to the oxidation of the Fe(II) cofactor to Fe(III). In this...

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Autori principali: Okuta, Akiko, Ohnishi, Kouhei, Harayama, Shigeaki
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC368311/
https://ncbi.nlm.nih.gov/pubmed/15006807
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.3.1804-1810.2004
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