Heterologous expression and characterization of the human R-ras gene product.

We directly expressed human R-ras 23,000-dalton protein (p23) cDNA in Escherichia coli under the control of the trp promoter. GTP-dependent phosphorylation of a p23 threonine 85 substitution mutant was observed. This result is in direct analogy to the autokinase activity of H-ras and K-ras threonine...

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Bibliographic Details
Main Authors: Lowe, D G, Goeddel, D V
Format: Artigo
Language:Inglês
Published: 1987
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Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC367902/
https://ncbi.nlm.nih.gov/pubmed/3313005
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