Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH

Human Dopa decarboxylase (hDDC), a pyridoxal 5′-phosphate (PLP) enzyme, displays maxima at 420 and 335 nm and emits fluorescence at 384 and 504 nm upon excitation at 335 nm and at 504 nm when excited at 420 nm. Absorbance and fluorescence titrations of hDDC-bound coenzyme identify a single pK(spec)...

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Main Authors: Montioli, Riccardo, Cellini, Barbara, Dindo, Mirco, Oppici, Elisa, Voltattorni, Carla Borri
Formato: Artigo
Idioma:Inglês
Publicado: Hindawi Publishing Corporation 2013
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Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3677616/
https://ncbi.nlm.nih.gov/pubmed/23781496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2013/161456
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