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Recorded Scan Times Can Limit the Accuracy of Sedimentation Coefficients in Analytical Ultracentrifugation

We report systematic and large inaccuracies in the recorded elapsed time in data files from the analytical ultracentrifuge, leading to overestimates of the sedimentation coefficients of up to 10%. This far exceeds previously considered factors contributing to the uncertainty in this parameter, and h...

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Hlavní autoři: Zhao, Huaying, Ghirlando, Rodolfo, Piszczek, Grzegorz, Curth, Ute, Brautigam, Chad A., Schuck, Peter
Médium: Artigo
Jazyk:Inglês
Vydáno: 2013
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3676908/
https://ncbi.nlm.nih.gov/pubmed/23458356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.02.011
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