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Recorded Scan Times Can Limit the Accuracy of Sedimentation Coefficients in Analytical Ultracentrifugation

We report systematic and large inaccuracies in the recorded elapsed time in data files from the analytical ultracentrifuge, leading to overestimates of the sedimentation coefficients of up to 10%. This far exceeds previously considered factors contributing to the uncertainty in this parameter, and h...

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Main Authors: Zhao, Huaying, Ghirlando, Rodolfo, Piszczek, Grzegorz, Curth, Ute, Brautigam, Chad A., Schuck, Peter
Format: Artigo
Jezik:Inglês
Izdano: 2013
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3676908/
https://ncbi.nlm.nih.gov/pubmed/23458356
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2013.02.011
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