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Use of a novel S1 nuclease RNA-mapping technique to measure efficiency of transcription termination on polyomavirus DNA.

We devised a strategy to measure the efficiency of transcription termination in vivo by RNA polymerase on polyomavirus DNA. Pulse-labeled nuclear RNA was hybridized with a single-stranded polyomavirus DNA fragment which spans the transcription initiation region. Hybrids were treated with RNase, boun...

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Bibliografske podrobnosti
Main Authors: Tseng, R W, Acheson, N H
Format: Artigo
Jezik:Inglês
Izdano: 1986
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC367689/
https://ncbi.nlm.nih.gov/pubmed/3023898
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