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Use of a novel S1 nuclease RNA-mapping technique to measure efficiency of transcription termination on polyomavirus DNA.
We devised a strategy to measure the efficiency of transcription termination in vivo by RNA polymerase on polyomavirus DNA. Pulse-labeled nuclear RNA was hybridized with a single-stranded polyomavirus DNA fragment which spans the transcription initiation region. Hybrids were treated with RNase, boun...
Enregistré dans:
| Publié dans: | Mol Cell Biol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Taylor & Francis
1986
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367689/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3023898/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.5.1624 |
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