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Use of a novel S1 nuclease RNA-mapping technique to measure efficiency of transcription termination on polyomavirus DNA.

We devised a strategy to measure the efficiency of transcription termination in vivo by RNA polymerase on polyomavirus DNA. Pulse-labeled nuclear RNA was hybridized with a single-stranded polyomavirus DNA fragment which spans the transcription initiation region. Hybrids were treated with RNase, boun...

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Detalles Bibliográficos
Publicado en:Mol Cell Biol
Main Authors: Tseng, R W, Acheson, N H
Formato: Artigo
Idioma:Inglês
Publicado: Taylor & Francis 1986
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367689/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3023898/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.5.1624
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