Transfer and amplification of a mutant beta-tubulin gene results in colcemid dependence: use of the transformant to demonstrate regulation of beta-tubulin subunit levels by protein degradation.

Total genomic DNA from a temperature-sensitive, colcemid-resistant Chinese hamster ovary (CHO) cell mutant expressing an electrophoretic variant beta-tubulin was used to transform wild-type CHO cells to colcemid-resistant cells at 37 degrees C. Southern blot analysis of the transformant demonstrated...

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Bibliographische Detailangaben
Veröffentlicht in:Mol Cell Biol
Hauptverfasser: Whitfield, C, Abraham, I, Ascherman, D, Gottesman, M M
Format: Artigo
Sprache:Inglês
Veröffentlicht: Taylor & Francis 1986
Schlagworte:
Research Article
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367666/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3785170/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.5.1422
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