Transfer and amplification of a mutant beta-tubulin gene results in colcemid dependence: use of the transformant to demonstrate regulation of beta-tubulin subunit levels by protein degradation.

Total genomic DNA from a temperature-sensitive, colcemid-resistant Chinese hamster ovary (CHO) cell mutant expressing an electrophoretic variant beta-tubulin was used to transform wild-type CHO cells to colcemid-resistant cells at 37 degrees C. Southern blot analysis of the transformant demonstrated...

Celý popis

Uloženo v:
Podrobná bibliografie
Hlavní autoři: Whitfield, C, Abraham, I, Ascherman, D, Gottesman, M M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1986
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC367666/
https://ncbi.nlm.nih.gov/pubmed/3785170
Tagy: Přidat tag
Žádné tagy, Buďte první, kdo otaguje tento záznam!

Podobné jednotky