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Dynamic Structure of the Translocon SecYEG in Membrane: DIRECT SINGLE MOLECULE OBSERVATIONS

Purified SecYEG was reconstituted into liposomes and studied in near-native conditions using atomic force microscopy. These SecYEG proteoliposomes were active in translocation assays. Changes in the structure of SecYEG as a function of time were directly visualized. The dynamics observed were signif...

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Autori principali: Sanganna Gari, Raghavendar Reddy, Frey, Nathan C., Mao, Chunfeng, Randall, Linda L., King, Gavin M.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Biochemistry and Molecular Biology 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3675617/
https://ncbi.nlm.nih.gov/pubmed/23609442
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M113.471870
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