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Direct imaging of single UvrD helicase dynamics on long single-stranded DNA

Fluorescence imaging of single-protein dynamics on DNA has been largely limited to double-stranded DNA or short single-stranded DNA. We have developed a hybrid approach for observing single proteins moving on laterally stretched kilobase-sized ssDNA. Here we probed the single-stranded DNA translocas...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Lee, Kyung Suk, Balci, Hamza, Jia, Haifeng, Lohman, Timothy M., Ha, Taekjip
Format: Artigo
Sprache:Inglês
Veröffentlicht: Nature Pub. Group 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3674262/
https://ncbi.nlm.nih.gov/pubmed/23695672
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/ncomms2882
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