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Comparison of LTQ-Orbitrap Velos and Q-Exactive for proteomic analysis of 1-1000 ng RAW 264.7 cell lysate digests

RATIONAL: There is interest in extending bottom-up proteomics to the smallest possible sample size. We investigated the performance of two modern instruments for the analysis of samples ranging from 1 ng to 1 µg of RAW 264.7 cell lysate digests. METHODS: A UPLC system coupled with either an LTQ-Orbi...

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Detalhes bibliográficos
Main Authors: Sun, Liangliang, Zhu, Guijie, Dovichi, Norman J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3673017/
https://ncbi.nlm.nih.gov/pubmed/23239329
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/rcm.6437
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