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Isolation of primitive endoderm, mesoderm, vascular endothelial and trophoblast progenitors from human pluripotent stem cells

To identify early populations of committed progenitors derived from human embryonic stem cells (hESCs), we screened self-renewing, BMP4-treated and retinoic acid–treated cultures with >400 antibodies recognizing cell-surface antigens. Sorting of >30 subpopulations followed by transcriptional a...

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Detalhes bibliográficos
Main Authors: Drukker, Micha, Tang, Chad, Ardehali, Reza, Rinkevich, Yuval, Seita, Jun, Lee, Andrew S., Mosley, Adriane R., Weissman, Irving L., Soen, Yoav
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3672406/
https://ncbi.nlm.nih.gov/pubmed/22634564
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.2239
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