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In Vivo Detection and Quantification of Chemicals that Enhance Protein Stability

We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. Addition of osmolytes and other compounds with chemical chaperone activity to the growth media of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent man...

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Detalhes bibliográficos
Main Authors: Hailu, Tsinatkeab T., Foit, Linda, Bardwell, James C. A.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3670414/
https://ncbi.nlm.nih.gov/pubmed/23219982
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.11.022
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