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In Vivo Detection and Quantification of Chemicals that Enhance Protein Stability
We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. Addition of osmolytes and other compounds with chemical chaperone activity to the growth media of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent man...
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Main Authors: | , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2012
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3670414/ https://ncbi.nlm.nih.gov/pubmed/23219982 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2012.11.022 |
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