Introduction of UAG, UAA, and UGA nonsense mutations at a specific site in the Escherichia coli chloramphenicol acetyltransferase gene: use in measurement of amber, ochre, and opal suppression in mammalian cells.

We have used oligonucleotide-directed site-specific mutagenesis to convert serine codon 27 of the Escherichia coli chloramphenicol acetyltransferase (cat) gene to UAG, UAA, and UGA nonsense codons. The mutant cat genes, under transcriptional control of the Rous sarcoma virus long terminal repeat, we...

Volledige beschrijving

Bibliografische gegevens

Gepubliceerd in:	Mol Cell Biol
Hoofdauteurs:	Capone, J P, Sedivy, J M, Sharp, P A, RajBhandary, U L
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	Taylor & Francis 1986
Onderwerpen:	Research Article
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367040/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3023959/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.9.3059-3067.1986
Tags:	Voeg label toe Geen labels, Wees de eerste die dit record labelt!