Introduction of UAG, UAA, and UGA nonsense mutations at a specific site in the Escherichia coli chloramphenicol acetyltransferase gene: use in measurement of amber, ochre, and opal suppression in mammalian cells.

We have used oligonucleotide-directed site-specific mutagenesis to convert serine codon 27 of the Escherichia coli chloramphenicol acetyltransferase (cat) gene to UAG, UAA, and UGA nonsense codons. The mutant cat genes, under transcriptional control of the Rous sarcoma virus long terminal repeat, we...

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Détails bibliographiques
Publié dans:Mol Cell Biol
Auteurs principaux: Capone, J P, Sedivy, J M, Sharp, P A, RajBhandary, U L
Format: Artigo
Langue:Inglês
Publié: Taylor & Francis 1986
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC367040/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3023959/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.6.9.3059
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