Biochemical properties of a highly purified v-rasH p21 protein overproduced in Escherichia coli and inhibition of its activities by a monoclonal antibody.

The v-rasH oncogene of Harvey murine sarcoma virus encodes a 21,000-dalton p21 protein which has been expressed at a high level as a fusion protein in Escherichia coli. We have purified the p21 to over 90% in purity without the use of any detergent or protein denaturant. The purified p21 possesses f...

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Bibliographic Details
Main Authors: Hattori, S, Ulsh, L S, Halliday, K, Shih, T Y
Format: Artigo
Language:Inglês
Published: 1985
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC366876/
https://ncbi.nlm.nih.gov/pubmed/3162096
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