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Purification, crystallization and preliminary crystallographic analysis of the 23S rRNA methyltransferase RlmM (Cm2498) from Escherichia coli

RlmM is an AdoMet-dependent methyltransferase that is responsible for 2′-­O-methylation of C2498 in the peptidyl-transferase loop of bacterial 23S rRNA. This modification occurs before assembly of the 50S ribosomal subunit, and lack of C2498 methylation can cause a slight reduction in bacterial fitn...

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Detalhes bibliográficos
Main Authors: Guo, Hong-Yue, Gao, Zeng-Qiang, Zhang, Heng, Wei, Yong, Xu, Jian-Hua, Wang, Wen-Ya, Yan, Ai-xia, Dong, Yu-Hui
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3668582/
https://ncbi.nlm.nih.gov/pubmed/23722841
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113006611
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