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Fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time PCR assay

BACKGROUND: A crucial step in the evaluation of newly produced transgenic plants is the selection of homozygous plants. Here we describe an efficient and highly flexible real-time PCR-based method for the development of homozygous lines in plant models with complex (multiple) genomes and/or relative...

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Autors principals: Mieog, Jos C, Howitt, Crispin A, Ral, Jean-Philippe
Format: Artigo
Idioma:Inglês
Publicat: BioMed Central 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3668147/
https://ncbi.nlm.nih.gov/pubmed/23627847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2229-13-71
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