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Fast and Efficient Screening for Wheat Loss-of-Gene Mutants Using Multiplexed Melt Curve Analyses

This study describes a new approach in the screening for loss-of-gene mutants in Heavy Ion Bombardment (HIB) mutant populations of genetically complex organisms such as hexaploid bread wheat using multiplexed single-color (SYBR Green) melt curve analyses. The assay was set up for three target genes...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Mieog, Jos C., Ral, Jean-Philippe F.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4961375/
https://ncbi.nlm.nih.gov/pubmed/27459606
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0159955
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