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RNA sequence containing hexanucleotide AAUAAA directs efficient mRNA polyadenylation in vitro.

To determine whether a specific nucleotide sequence is required to direct polyadenylation of a simian virus 40 early pre-mRNA in a soluble HeLa whole-cell lysate, we constructed a series of rearranged and deleted DNA templates, transcribed them in vitro, and determined whether the resultant RNAs cou...

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Bibliografiske detaljer
Udgivet i:Mol Cell Biol
Main Authors: Manley, J L, Yu, H, Ryner, L
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 1985
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC366720/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2579321/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.5.2.373
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