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Alternative Sigma Factor Over-Expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia coli

BACKGROUND: Heterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways. Development of an effective, general heterologous expression system that can be applied to bioprospecting from metagenomic DNA will enable the disc...

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Autori principali: Stevens, David Cole, Conway, Kyle R., Pearce, Nelson, Villegas-Peñaranda, Luis Roberto, Garza, Anthony G., Boddy, Christopher N.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3665592/
https://ncbi.nlm.nih.gov/pubmed/23724102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0064858
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