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Alternative Sigma Factor Over-Expression Enables Heterologous Expression of a Type II Polyketide Biosynthetic Pathway in Escherichia coli

BACKGROUND: Heterologous expression of bacterial biosynthetic gene clusters is currently an indispensable tool for characterizing biosynthetic pathways. Development of an effective, general heterologous expression system that can be applied to bioprospecting from metagenomic DNA will enable the disc...

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Detalhes bibliográficos
Main Authors: Stevens, David Cole, Conway, Kyle R., Pearce, Nelson, Villegas-Peñaranda, Luis Roberto, Garza, Anthony G., Boddy, Christopher N.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3665592/
https://ncbi.nlm.nih.gov/pubmed/23724102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0064858
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