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Inferring subunit stoichiometry from single molecule photobleaching

Single molecule photobleaching is a powerful tool for determining the stoichiometry of protein complexes. By attaching fluorophores to proteins of interest, the number of associated subunits in a complex can be deduced by imaging single molecules and counting fluorophore photobleaching steps. Becaus...

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Detalles Bibliográficos
Autor Principal: Hines, Keegan E.
Formato: Artigo
Idioma:Inglês
Publicado: The Rockefeller University Press 2013
Assuntos:
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664702/
https://ncbi.nlm.nih.gov/pubmed/23712552
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1085/jgp.201310988
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