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Measuring the Stoichiometry of Functional PspA Complexes in Living Bacterial Cells by Single Molecule Photobleaching
We report a general method based on wide-field fluorescence imaging of single molecule photobleaching and Chung-Kennedy algorithm to measure the stoichiometry of functional protein complexes in living bacterial cells.
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3337751/ https://ncbi.nlm.nih.gov/pubmed/20830338 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1039/c0cc01707h |
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