PCR on yeast colonies: an improved method for glyco-engineered Saccharomyces cerevisiae

BACKGROUND: Saccharomyces cerevisiae is extensively used in bio-industries. However, its genetic engineering to introduce new metabolism pathways can cause unexpected phenotypic alterations. For example, humanisation of the glycosylation pathways is a high priority pharmaceutical industry goal for p...

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Bibliographic Details
Main Authors: Bonnet, Christine, Rigaud, Céline, Chanteclaire, Emilie, Blandais, Claire, Tassy-Freches, Emilie, Arico, Christelle, Javaud, Christophe
Format: Artigo
Language:Inglês
Published: BioMed Central 2013
Subjects:
Technical Note
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3664073/
https://ncbi.nlm.nih.gov/pubmed/23688076
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-6-201
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