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Crystallization and preliminary X-ray analysis of the CRP–cAMP–DNA (full length) complex

The Escherichia coli cyclic AMP receptor protein (CRP) is a well known transcription activator protein. In this study, CRP was overexpressed, purified and cocrystallized with cAMP and a 38 bp full-length double-stranded DNA fragment. The full-length segment differed from the half-site fragments used...

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Bibliografiske detaljer
Main Authors: Huang, Jing, Liu, Jing, Tao, Wenbing, Yang, Zhenxing, Qiu, Rui, Yu, Shaoning, Ji, Chaoneng
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3660902/
https://ncbi.nlm.nih.gov/pubmed/23695578
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113009925
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