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Crystallization and preliminary X-ray analysis of the ligand-binding domain of cAMP receptor protein

The cyclic AMP receptor protein (CRP) from Escherichia coli regulates the expression of a large number of genes. In this work, CRP has been overexpressed, purified and digested by subtilisin and chymotrypsin. The fragments S-CRP (digested by subtilisin) and CH-CRP (digested by chymotrypsin) have bee...

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Hlavní autoři: Tao, Wenbing, Li, Feng, Liu, Haiping, Bao, Xiangyu, Gong, Weimin, Yu, Shaoning
Médium: Artigo
Jazyk:Inglês
Vydáno: International Union of Crystallography 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2864681/
https://ncbi.nlm.nih.gov/pubmed/20445248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110008675
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