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Triosephosphate isomerase is a common crystallization contaminant of soluble His-tagged proteins produced in Escherichia coli

Attempts to crystallize several mammalian proteins overexpressed in Escherichia coli revealed a common contaminant, triosephosphate isomerase, a protein involved in glucose metabolism. Even with triosephosphate isomerase present in very small amounts, similarly shaped crystals appeared in the crysta...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Kozlov, Guennadi, Vinaik, Roohi, Gehring, Kalle
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2013
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3660886/
https://ncbi.nlm.nih.gov/pubmed/23695562
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113010841
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