Adaptation to Robust Monolayer Expansion Produces Human Pluripotent Stem Cells With Improved Viability

The generation of human pluripotent stem cells (hPSCs) of sufficient quantity and quality remains a major challenge for biomedical application. Here we present an efficient feeder-free, high-density monolayer system in which hPSCs become SSEA-3-high and gradually more viable than their feeder-depend...

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Bibliografske podrobnosti
Main Authors: Kunova, Michaela, Matulka, Kamil, Eiselleova, Livia, Salykin, Anton, Kubikova, Iva, Kyrylenko, Sergiy, Hampl, Ales, Dvorak, Petr
Format: Artigo
Jezik:Inglês
Izdano: AlphaMed Press 2013
Teme:
Embryonic Stem Cells/Induced Pluripotent Stem (iPS) Cells
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC3659836/
https://ncbi.nlm.nih.gov/pubmed/23486835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.5966/sctm.2012-0081
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