Spontaneous nucleotide exchange in low molecular weight GTPases by fluorescently labeled γ-phosphate-linked GTP analogs

Regulated guanosine nucleotide exchange and hydrolysis constitute the fundamental activities of low molecular weight GTPases. We show that three guanosine 5′-triphosphate analogs with BODIPY fluorophores coupled via the gamma phosphate bind to the GTPases Cdc42, Rac1, RhoA, and Ras and displace guan...

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Detaylı Bibliyografya
Asıl Yazarlar: Korlach, Jonas, Baird, Daniel W., Heikal, Ahmed A., Gee, Kyle R., Hoffman, Gregory R., Webb, Watt W.
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: National Academy of Sciences 2004
Konular:
Biological Sciences
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC365700/
https://ncbi.nlm.nih.gov/pubmed/14973186
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0308579100
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