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Single molecule pull-down for studying protein interactions
This protocol describes a single molecule pull-down (SiMPull) assay for analyzing physiological protein complexes. The assay combines the conventional pull-down assay with single molecule total internal reflection fluorescence microscopy, and allows probing single macromolecular complexes directly f...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2012
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3654178/ https://ncbi.nlm.nih.gov/pubmed/22322217 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2011.452 |
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