Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants.

A method for introducing specific mutations into the diploid Candida albicans by one-step gene disruption and subsequent UV-induced recombination was developed. The cloned C. albicans URA3 gene was disrupted with the C. albicans ADE2 gene, and the linearized DNA was used for transformation of two ad...

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Bibliografiske detaljer

Udgivet i:	Mol Cell Biol
Principais autores:	Kelly, R, Miller, S M, Kurtz, M B, Kirsch, D R
Format:	Artigo
Sprog:	Inglês
Udgivet:	Taylor & Francis 1987
Fag:	Research Article
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC365057/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031459/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.7.1.199-208.1987
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