Directed mutagenesis in Candida albicans: one-step gene disruption to isolate ura3 mutants.
A method for introducing specific mutations into the diploid Candida albicans by one-step gene disruption and subsequent UV-induced recombination was developed. The cloned C. albicans URA3 gene was disrupted with the C. albicans ADE2 gene, and the linearized DNA was used for transformation of two ad...
Guardat en:
| Publicat a: | Mol Cell Biol |
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| Autors principals: | , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
1987
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC365057/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3031459/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.7.1.199-208.1987 |
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