Microscale Thermophoresis Quantifies Biomolecular Interactions under Previously Challenging Conditions

Microscale thermophoresis (MST) allows for quantitative analysis of protein interactions in free solution and with low sample consumption. The technique is based on thermophoresis, the directed motion of molecules in temperature gradients. Thermophoresis is highly sensitive to all types of binding-i...

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Main Authors: Seidel, Susanne A.I., Dijkman, Patricia M., Lea, Wendy A., van den Bogaart, Geert, Jerabek-Willemsen, Moran, Lazic, Ana, Joseph, Jeremiah S., Srinivasan, Prakash, Baaske, Philipp, Simeonov, Anton, Katritch, Ilia, Melo, Fernando A., Ladbury, John E., Schreiber, Gideon, Watts, Anthony, Braun, Dieter, Duhr, Stefan
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3644557/
https://ncbi.nlm.nih.gov/pubmed/23270813
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.12.005
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