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SDM-Assist software to design site-directed mutagenesis primers introducing “silent” restriction sites
BACKGROUND: Over the past decades site-directed mutagenesis (SDM) has become an indispensable tool for biological structure-function studies. In principle, SDM uses modified primer pairs in a PCR reaction to introduce a mutation in a cDNA insert. DpnI digestion of the reaction mixture is used to eli...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2013
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3644487/ https://ncbi.nlm.nih.gov/pubmed/23522286 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-14-105 |
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