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Application of the Amplex Red/Horseradish Peroxidase Assay to Measure Hydrogen Peroxide Generation by Recombinant Microsomal Enzymes
The formation of reactive oxygen species by the cytochrome P450 monoxygenase system is thought to be due to autooxidation of NADPH-cytochrome P450 reductase and the non-productive decay of oxygen-bound cytochrome P450 intermediates. To characterize this process in recombinant microsomal enzymes, we...
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| Main Authors: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3643635/ https://ncbi.nlm.nih.gov/pubmed/20188819 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.freeradbiomed.2010.02.030 |
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