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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy

Cells can sense and migrate towards higher concentrations of adhesive cues such as the glycoproteins of the extracellular matrix and soluble cues such as growth factors. Here, we outline a method to create opposing gradients of adhesive and soluble cues in a microfluidic chamber, which is compatible...

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Bibliografski detalji
Glavni autori: Ngalim, Siti Hawa, Magenau, Astrid, Zhu, Ying, Tønnesen, Lotte, Fairjones, Zoe, Gooding, J. Justin, Böcking, Till, Gaus, Katharina
Format: Artigo
Jezik:Inglês
Izdano: MyJove Corporation 2013
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3643214/
https://ncbi.nlm.nih.gov/pubmed/23609313
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50310
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