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Creating Adhesive and Soluble Gradients for Imaging Cell Migration with Fluorescence Microscopy
Cells can sense and migrate towards higher concentrations of adhesive cues such as the glycoproteins of the extracellular matrix and soluble cues such as growth factors. Here, we outline a method to create opposing gradients of adhesive and soluble cues in a microfluidic chamber, which is compatible...
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| Main Authors: | , , , , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3643214/ https://ncbi.nlm.nih.gov/pubmed/23609313 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50310 |
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