A quantitative study of methanol/sorbitol co-feeding process of a Pichia pastoris Mut(+)/pAOX1-lacZ strain

BACKGROUND: One of the main challenges for heterologous protein production by the methylotrophic yeast Pichia pastoris at large-scale is related to its high oxygen demand. A promising solution is a co-feeding strategy based on a methanol/sorbitol mixture during the induction phase. Nonetheless, a de...

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Hlavní autoři: Niu, Hongxing, Jost, Laurent, Pirlot, Nathalie, Sassi, Hosni, Daukandt, Marc, Rodriguez, Christian, Fickers, Patrick
Médium: Artigo
Jazyk:Inglês
Vydáno: BioMed Central 2013
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Research
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3639866/
https://ncbi.nlm.nih.gov/pubmed/23565774
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1475-2859-12-33
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