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Single-molecule Imaging of Gene Regulation In vivo Using Cotranslational Activation by Cleavage (CoTrAC)
We describe a fluorescence microscopy method, Co-Translational Activation by Cleavage (CoTrAC) to image the production of protein molecules in live cells with single-molecule precision without perturbing the protein's functionality. This method makes it possible to count the numbers of protein...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
MyJove Corporation
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3639517/ https://ncbi.nlm.nih.gov/pubmed/23524866 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50042 |
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