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Fluorescence imaging of single-copy DNA sequences within the human genome using PNA-directed padlock probe assembly
We present a novel approach for fluorescent in situ detection of short, single-copy sequences within genomic DNA in human cells. The single copy sensitivity and single base specificity of our method is achieved due to the combination of three components. First, a peptide nucleic acid (PNA) probe loc...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3636569/ https://ncbi.nlm.nih.gov/pubmed/23521801 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.chembiol.2013.02.012 |
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