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Integrating de novo Sequencing and Database Search for Monoclonal Antibody Sequencing

A mass spectrometry-based workflow for sequencing the monoclonal antibodies was presented. It combined multi-enzyme digestion, acquisition of high-resolution on both precursor and fragment steps, and integration of database search and de novo sequencing. All MS/MS spectra were first searched against...

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Detalhes bibliográficos
Main Authors: Xin, Lei, Shan, Baozhen
Formato: Artigo
Idioma:Inglês
Publicado em: Association of Biomolecular Resource Facilities 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635332/
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