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Direct Sequencing of Linear DNA on PacBio RS Without Library Preparation
All nextGen sequencing technologies require library construction as a first step where platform-specific adaptors are ligated to target DNA fragments. Pacific Biosciences RS technology requires 5-10ug of DNA for large insert (∼10kb) library construction. However, under circumstances such as when per...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Association of Biomolecular Resource Facilities
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3635274/ |
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