Direct Sequencing of Linear DNA on PacBio RS Without Library Preparation

All nextGen sequencing technologies require library construction as a first step where platform-specific adaptors are ligated to target DNA fragments. Pacific Biosciences RS technology requires 5-10ug of DNA for large insert (∼10kb) library construction. However, under circumstances such as when per...

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Autors principals: Zhou, Xiao H., Holland, Kevin, Moraga, David, Almira, Ernesto C., Farmerie, Willam G., Shanker, Savita
Format: Artigo
Idioma:Inglês
Publicat: Association of Biomolecular Resource Facilities 2013
Matèries:
Poster Session Abstracts
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3635274/
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