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piggyBac as a high-capacity transgenesis and gene-therapy vector in human cells and mice

The stable genomic integration and expression of a large transgene is a major hurdle in gene therapy. We show that the modified piggyBac (PB) transposon system can be used to introduce a 207 kb genomic DNA fragment containing the RORγ/γt locus into human cells and mice. PB-mediated transgenesis resu...

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Bibliografische gegevens
Hoofdauteurs: Li, Rongbo, Zhuang, Yuan, Han, Min, Xu, Tian, Wu, Xiaohui
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: The Company of Biologists Limited 2013
Onderwerpen:
Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3634665/
https://ncbi.nlm.nih.gov/pubmed/23519027
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dmm.010827
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