Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC)

Resolving single fluorescent molecules in the presence of high fluorophore concentrations remains a challenge in single-molecule biophysics that limits our understanding of weak molecular interactions. Total internal reflection fluorescence (TIRF) imaging, the workhorse of single-molecule fluorescen...

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Hlavní autoři: Elting, Mary Williard, Leslie, Sabrina R., Churchman, L. Stirling, Korlach, Jonas, McFaul, Christopher M. J., Leith, Jason S., Levene, Michael J., Cohen, Adam E., Spudich, James A.
Médium: Artigo
Jazyk:Inglês
Vydáno: Optical Society of America 2013
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Research-Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3632498/
https://ncbi.nlm.nih.gov/pubmed/23389011
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/OE.21.001189
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